effect of intracerebroventricular injection of olive leaf extract on ptz-induced seizures in male rats

introduction: olive leaf contains anti-inflammatory and cox-2 inhibitor compounds. considering the role of the cox enzyme in seizures, this research was performed to assess the effects of this extract in treatment of seizures induced by ptz. methods: in this research, male wistar rats (200±20 g) were injected intra-cerebroventricularly with 1 μl saline or olive leaf extract (125 μg, 250 μg and 500 μg), before the intraperitoneal administration of ptz (80 mg/kg) for induction of seizure. then, seizure score and onset times of every stage of seizure were recorded during 20 minutes after ptz administration. the data was analyzed by one-way analysis of variance (anova) and non parametric tests. results: result of this research showed that the injection of 125, 250 and 500 μg of olive leaf extract had no effect on the onset time of seizure stages 1, 2 and 3 compared to the control group (p>0.05). however, they significantly decreased the onset time of seizure stages 4 and 5 compared to the control group (p<0.05). on the other hand, olive leaf extract decreased onset time of generalized (tonic-clonic) seizures, but had no effect on partial seizures. different doses of olive leaf extract had no effect on seizure scores. conclusion: olive leaf extract has convulsive property and this effect is time and dose dependent.